Dynabeads™ Oligo(dT)₂₅ mRNA isolation beads specifically target and capture virtually any crude sample When the required information-containing nucleic acid is mRNA, there is no need to purify total RNA. Since mRNA only accounts for approximately 1–5% of total cellular RNA, total RNA isolation is not the most efficient method for isolating mRNA. Other techniques designed to purify total RNA yield ∼80% ribosomal RNA and force the mRNA to compete for membrane binding with ribosomal RNA, transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA. Advantages of Dynabeads™ Oligo(dT)₂₅ beads:

• Complete and pure mRNA can be obtained through fast and gentle procedures
• Ultra-pure mRNA isolation, upstream of cDNA synthesis Excellent choice
• Extremely sensitive mRNA isolation enables cDNA synthesis and cDNA library construction from ultra-small input samples (cDNA library construction from single cells)

How Beads Work
Oligonucleotide (dT)₂₅-coated Dynabeads™ specifically target and capture the mRNA transcriptome from an extremely broad range of crude starting samples. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, microRNA, and small nucleolar RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured. The isolated mRNA is pure and does not require ribosomal RNA reduction or post-extraction DNase treatment. This column-free system ensures extremely high transcriptome recovery:

• Physical mRNA capture on moving magnetic beads
• Fast and gentle magnetic processing
• No loss of energy during high-speed centrifugation Loss of mRNA
• During elution, mRNA does not adsorb to the column membrane

Applications
mRNA is suitable for all downstream molecular applications, including gene cloning, cDNA synthesis , cDNA library construction, RT-PCR quantitative RT-PCR, RPA (ribonuclease protection assay), subtractive hybridization, primer extension, SAGE, RACE and others. Dynabeads™ Oligo(dT)₂₅ mRNA Isolation Beads are an ideal method for purifying mRNA prior to cDNA library construction. The use of these beads ensures extremely high recovery and enrichment of the transcriptome. By incorporating a total RNA isolation step upstream of mRNA isolation, these beads may potentially capture a larger portion of the transcriptome.

Verastile elution options
Elute at any volume as low as 5 µL. Elution of mRNA is optional as the presence of Dynabeads™ does not inhibit enzymatic reactions in downstream procedures. Additionally, cDNA synthesis can be performed directly on beads to create reusable solid-phase cDNA libraries.